مقایسه کارگرد پادگنهای تهیه شده از B. abortus و B. melitensis در نتایج آزمایشهای سرولوژیک در گوسفند و بز

Authors

  • فرامرز طاهری دانشکده دامپزشکی دانشگاه شیراز
  • مرتضی ذوالانواری دانشکده دامپزشکی دانشگاه شیراز
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A serological comparison of complement fixation reactions using Brucella abortus and B. melitensis antigens in B. abortus infected cattle.

Brucella abortus and B. melitensis antigens were used in parallel on the National Standard Brucella abortus antiserum and on field sera coming from cattle where practically exclusively B. abortus biotypes 1 and 2 have been isolated over the last 11 years. With the National Standard serum the titres to B. melitensis were consistently lower than those to B. abortus antigen. Most were 1 dilution (...

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Real-time multiplex PCR assay for detection of Brucella spp., B. abortus, and B. melitensis.

The identification of Brucella can be a time-consuming and labor-intensive process that places personnel at risk for laboratory-acquired infection. Here, we describe a real-time PCR assay for confirmation of presumptive Brucella isolates. The assay was designed in a multiplex format that will allow the rapid identification of Brucella spp., B. abortus, and B. melitensis in a single test.

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Journal title

volume 9  issue 2

pages  77- 79

publication date 1996-06-21

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